FIG 2.

Inflammatory responses in nascent abscesses. C57BL/6J mice were infected in the subcutaneous space with strain PA99. (A) Abscess tissues were harvested and homogenized at the indicated times postinfection, and IL-1β levels were determined by ELISA. Data are means plus standard errors of the means. Experiments were done on three mice per group and were performed at least twice. Statistical analyses were performed by a one-way ANOVA and Dunnett's multiple-comparison test. ***, P < 0.001 compared to day 1. (B) Abscess tissues were harvested and homogenized at 24 h postinfection, and myeloperoxidase activity was measured as a surrogate for neutrophil activity. Experiments were done on five mice per group. Data are means plus standard deviations. Data were analyzed by an unpaired t test. ***, P < 0.001. D.P.I., days postinfection; PBS, phosphate-buffered saline; MPO, myeloperoxidase.