FIG 4.

Roles of type III secretion effector proteins in abscess formation. C57BL/6J mice were infected in the subcutaneous space with P. aeruginosa strains lacking either a single type III effector (A and B) or two type III effectors (C and D). (A, C) Abscess sizes were measured over a 2-week period, and the peak abscess sizes at 24 h postinfection (from panels A and C) were plotted in bar graphs (B and D). The data in panels A and C represent mean abscess areas at each time point, and error bars represent standard errors of the means. The data in panels B and D represent means plus standard errors of the means and were analyzed by one-way ANOVA and Dunnett's multiple-comparison test. *, P < 0.05; ***, P < 0.001. Experiments were done on five mice per group. D.P.I., days postinfection.