FIG 5.

Characteristics of abscesses in neutropenic mice. C57BL/6J mice were injected in the peritoneum with IgG2a control antibodies or anti-Ly6G antibodies to deplete the neutrophils. The mice were then infected in the subcutaneous space with P. aeruginosa strain PA99. (A, B) Abscess areas (A) and dermonecrotic areas (B) were measured daily following infection. (B, inset) Picture of a representative dermonecrotic skin lesion from an anti-Ly6G antibody-treated mouse. (C to E) Abscess (C), spleen (D), and liver (E) tissues were homogenized at 24 h postinfection and plated for CFU enumeration. The dashed lines represent the limit of detection. Data are means plus standard errors of the means. Experiments were done on three mice per group and were performed at least twice. Data were analyzed by unpaired t tests. *, P < 0.05; **, P < 0.01. D.P.I., days postinfection.