FIG 1.

Procedure for generating biofilm preparations for analysis and the parameters that were assessed in comparing the effects of extracts and compounds on individual biofilm development. (A) Flowchart of the procedure. See Materials and Methods and Results for details. (B) Model of a control biofilm and the assessed parameters. Note that pseudohyphae (parameter 7) are not formed in untreated control biofilms. SE disc, silicone elastomer disc; sLee's medium, supplemented Lee's medium; BYCP, basal yeast cell polylayer; CLSM, confocal laser scanning microscopy, GFP-expressing PMNs, green fluorescent protein-expressing polymorphonuclear leukocytes.