TABLE 5.
Summary of defects caused by 81 extracts on biofilm architecture and PMN impenetrability in the three thickness categoriesa
Category | No. | % of extracts in each category in which the following had a score of −, +, or ++: |
|||||||
---|---|---|---|---|---|---|---|---|---|
Presence of BYCP | Hypha formation | Vertical hypha orientation | ECM staining | PMN impenet. | Hyphal bend point | Pseudohypha formation | One or more affected parameters | ||
Thicker | 10 | 0 | 10 | 10 | 50 | 50 | 10 | 20 | 60 |
Thinner | 25 | 44 | 20 | 44 | 52 | 68 | 36 | 16 | 84 |
Similar | 46 | 10 | 4 | 20 | 33 | 37 | 9 | 7 | 52 |
Comparisons to the untreated control were made. No., number of extracts resulting in biofilms that were thicker than the control biofilm, thinner than the control biofilm, or similar in thickness to the control biofilm. BYCP, basal yeast cell polylayer; ECM staining, staining if extracellular matrix with calcofluor white; PMN impenet., polymorphonuclear leukocyte impenetrability; hyphal bend point, the proportion of extract-treated biofilms in a category in which the bend point occurred at less than 60% of the hyphal length.