FIG 6.

AfYap1 accumulates in the nucleus when A. fumigatus is stressed with amphotericin B but not when it is stressed with itraconazole or terbinafine. Microscopic images of the A. fumigatus yap1_eGFP strain. A total of 10⁴ spores of the yap1_eGFP strain were cultivated in AMM at 37°C overnight. Mitochondrial complex I was inhibited by the addition of 75 μM rotenone to the fungal mycelia 1 h before the treatment with stressors. We added 5 mM menadione, 4 mg/liter itraconazole (ITC), 4 mg/liter terbinafine (TRB), and 2.5 mg/liter amphotericin B (AMB) to A. fumigatus mycelia, followed by incubation for 45 min before fixation. Exposure to menadione was used as a positive control for oxidative stress. Hoechst 34580 (HOE)-stained nuclei are shown in blue.