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. 2017 Oct 24;6:e30057. doi: 10.7554/eLife.30057

Figure 2. daf-16/FoxO shifts metabolic flux and increases trehalose synthesis during starvation.

(A) Principal component analysis (PCA) of targeted metabolomic analysis fed and starved wild-type (WT) and daf-16 L1 larvae shows separation by condition, but not by genotype. 80% confidence ellipses are included with seven biological replicates. (B) Disaccharide levels were measured in non-targeted metabolomic analysis. Mean and SD of log2 fold-change relative to fed WT is plotted for four biological replicates (pint=0.05, n = 4, two-way ANOVA). (C) Trehalose levels were measured in a biochemical assay, and log2 fold-change relative to fed WT is plotted for four biological replicates (pint=0.003, n = 4, two-way ANOVA). (D) Trehalose content in fed (gray) and starved (orange) worms is plotted over time. Fed and starved conditions are significantly different (p=0.01, n = 2, two-way ANOVA). Mean and standard error of the mean of two biological replicates are shown.

Figure 2—source data 1. Raw data for display items in Figure 2.
elife-30057-fig2-data1.xlsx (177.5KB, xlsx)
DOI: 10.7554/eLife.30057.010

Figure 2.

Figure 2—figure supplement 1. Metabolomic analysis of WT and daf-16/FoxO worms during feeding and starvation.

Figure 2—figure supplement 1.

Targeted panels of metabolites are plotted as log2 fold-changes relative to the WT-fed condition. Metabolites profiled include (A) organic acids, (B) amino acids, (C) short and medium chain acyl carnitines (C2–C14), and (D) long-chain acyl carnitines (C16–C22).