Reagent type (species) or resource	Designation	Source or reference	Identifiers	Additional information
strain, strain background (C. elegans)	N2 (Bristol)	Caenorhabditis Genetics Center	N2
strain, strain background (C. elegans)	daf-16(mgDf50)	Caenorhabditis Genetics Center	RRID:WB-STRAIN: GR1307
strain, strain background (C. elegans)	daf-16(mgDf47)	Caenorhabditis Genetics Center	PS5150
strain, strain background (C. elegans)	dpy-5(e907); sEx14885	Caenorhabditis Genetics Center	RRID:WB-STRAIN: BC14885
strain, strain background (C. elegans)	dpy-5(e907); sEx14876	Caenorhabditis Genetics Center	RRID:WB-STRAIN: BC14876
strain, strain background (C. elegans)	ayIs7[Phlh-8::GFP]	Caenorhabditis Genetics Center	RRID:WB-STRAIN: PD4667
strain, strain background (C. elegans)	daf-16(mgDf50); ayIs7[Phlh-8::GFP]	As described in Baugh and Sternberg (2006) DOI: 10.1016/j.cub.2006.03.021, Kaplan et al. (2015) DOI:10.1371/journal.pgen.1005731	LRB101
strain, strain background (C. elegans)	icl-1(ok531)	Caenorhabditis Genetics Center	RRID:WB-STRAIN: RB766
strain, strain background (C. elegans)	pck-1(ok2098)	Caenorhabditis Genetics Center	RRID:WB-STRAIN: RB1688
strain, strain background (C. elegans)	pyk-1(ok1754)	Caenorhabditis Genetics Center	RRID:WB-STRAIN: VC1265
strain, strain background (C. elegans)	tps-1(ok373)	Caenorhabditis Genetics Center	RRID:WB-STRAIN: VC225
strain, strain background (C. elegans)	tps-2(ok526)	Caenorhabditis Genetics Center	RRID:WB-STRAIN :RB760
strain, strain background (C. elegans)	tps-1(ok373); tps-2(ok526)	Erkut et al. (2011) DOI: 10.1016/j.cub.2011.06.064		Shared by Teymuras Kurzchalia
strain, strain background (C. elegans)	tps-1(ok373); tps-2(ok526); daf-2(e1370)	Erkut et al. (2011) DOI: 10.1016/j.cub.2011.06.065		Shared by Teymuras Kurzchalia
strain, strain background (C. elegans)	tre-1(ok327)	Caenorhabditis Genetics Center	RRID:WB-STRAIN: RB728
strain, strain background (C. elegans)	tre-2(ok575)	Caenorhabditis Genetics Center	RRID:WB-STRAIN: RB789
strain, strain background (C. elegans)	tre-3(ok394)	Caenorhabditis Genetics Center	RRID:WB-STRAIN: RB1400
strain, strain background (C. elegans)	tre-4(gk298765)	Caenorhabditis Genetics Center	LRB327	2x backcrossed VC40057
strain, strain background (C. elegans)	tre-5(ok612)	Caenorhabditis Genetics Center	RRID:WB-STRAIN: RB806
strain, strain background (C. elegans)	tre-1(ok327); tre-5(ok612); tre-2(ok575); tre-3(ok394); tre-4(gk298765)	this paper	LRB334	This strain was generated by crosses to combine each of the individual trehalase mutants listed above.
strain, strain background (C. elegans)	dpy-5(e907); sEx14863	Caenorhabditis Genetics Center	RRID:WB-STRAIN: BC14863
strain, strain background (C. elegans)	dpy-5(e907); sIs11667	Caenorhabditis Genetics Center	RRID:WB-STRAIN: BC12475
strain, strain background (C. elegans)	dpy-5(e907); sEx15383	Caenorhabditis Genetics Center	RRID:WB-STRAIN: BC15383
strain, strain background (C. elegans)	dpy-5(e907); sEx14865	Caenorhabditis Genetics Center	RRID:WB-STRAIN: BC14865
strain, strain background (C. elegans)	syIs78[Pajm-1::AJM-1::GFP; unc-119+]	Caenorhabditis Genetics Center	RRID:WB-STRAIN: PS4657
strain, strain background (C. elegans)	daf-16(mgDf50); syIs78[Pajm-1::AJM-1::GFP]	As described in Baugh and Sternberg (2006) DOI: 10.1016/j.cub.2006.03.021, Kaplan et al. (2015) DOI:10.1371/journal.pgen.1005731	LRB240
strain, strain background (C. elegans)	tps-1(ok373); tps-2(ok526); daf-16(mgDf50); syIs78[Pajm-1::AJM-1::GFP]	this paper		Strain LRB240 was crossed into tps-1(ok373); tps-2(ok526)
commercial assay or kit	C. elegans expression array	Affymetrix	900383
commercial assay or kit	NEBNext Ultra RNA Library Prep Kit for Illumina	New England BioLabs	E7530
commercial assay or kit	Bicinchoninic acid kit	Sigma	BCA1
commercial assay or kit	Trehalose assay kit	Megazyme	K-TREH
commercial assay or kit	Qubit protein assay kit	Thermo Fisher Scientific	Q33211
chemical compound, drug	2-deoxy-D-glucose	Sigma	154-17-6
chemical compound, drug	3-mercaptopicolinic acid (3 MPA)	Santa Cruz Biotechnology	320386-54-7
chemical compound, drug	D-(+)-Trehalose dihydrate	Sigma	6138-23-4
chemical compound, drug	α,β-trehalose	Omicron Biochemicals Inc.	TRE-006
software, algorithm	WormSizer	Moore et al. (2013) DOI: 10.1371/journal.pone.0057142		https://github.com/bradtmoore/wormsizer