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. 2017 Oct 24;6:e29914. doi: 10.7554/eLife.29914

Figure 5. Surface Ca2+-dependent Cl- current in HEK293 cells expressing WT and mutant BEST1.

(A) Representative current traces recorded from transfected HEK293 cells at 1.2 μM [Ca2+]i. Scale bar, 150 pA, 150 ms. (B) Population steady-state current-voltage relationships for BEST1 WT (●), P274R (▲) and I201T (▼) at 1.2 μM [Ca2+]i; n = 5–6 for each point. ∗#p<0.05 compared to WT (8 × 10−4 for P274R and 0.01 for I201T) or to I201T (0.04), respectively, using one-way ANOVA and Bonferroni post hoc analyses. See also Figure 5—figure supplement 1.

Figure 5.

Figure 5—figure supplement 1. Ca2+-dependent Cl- current in BEST1 transfected HEK293 cells.

Figure 5—figure supplement 1.

(A) Representative current traces recorded from untransfected HEK293 cells at 1.2 μM [Ca2+]i. Scale bar, 150 pA, 150 ms. (B) Bar chart showing population steady-state current amplitudes at 100 mV; n = 5–6. ∗p<0.05 compared to current amplitudes at 1.2 μM [Ca2+]i in HEK293 cells transfected with BEST1 WT using two-tailed unpaired Student t test. (C) Western blot showing similar expression levels of transiently transfected BEST1 WT, P274R and I201T in HEK293 cells.