Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| gene (human) | BEST1 | PMID: 25324390 | ||
| gene (Klebsiella pneumoniae) |
KpBest | PMID: 25324390 | ||
| strain, strain background (E.coli) |
DH5alpha | other | Laboratory of Wayne Hendrickson |
|
| strain, strain background (E. coli) |
BL21 plysS | other | Laboratory of Wayne Hendrickson |
|
| cell line (human) | HEK293 | other | RRID:CVCL_0045 | Laboratory of David Yule |
| transfected construct (human) |
pEGFP-N1-BEST1 WT | PMID: 25324390 | ||
| transfected construct (human) |
pEGFP-N1-BEST1 I201T | this paper | Made from pEGFP-N1-BEST1 WT by site-directed mutagenesis |
|
| transfected construct (human) |
pEGFP-N1-BEST1 P274R | this paper | Made from pEGFP-N1-BEST1 WT by site-directed mutagenesis |
|
| biological sample (human) |
skin cells | other | New York Presbyterian Hospital |
|
| biological sample (human) |
fetus eye samples | other | New York Presbyterian Hospital |
|
| biological sample (human) |
BEST1 WT iPSC-RPE | this paper | Generated from donor skin cells by re-programming and differentiation |
|
| biological sample (human) |
BEST1 I201T iPSC-RPE | this paper | Generated from donor skin cells by re-programming and differentiation |
|
| biological sample (human) |
BEST1 P274R iPSC-RPE | this paper | Generated from donor skin cells by re-programming and differentiation |
|
| antibody | BESTROPHIN1 | Novus Biologicals NB300-164 | RRID:AB_10003019 | 1:200 |
| antibody | ZO-1 | Invitrogen 40–2200 | RRID:AB_2533456 | 1:500 |
| antibody | Alexa Fluor 488-conjugated IgG | Invitrogen A-11070 | RRID:AB_2534114 | 1:1000 |
| antibody | Alexa Fluor 555-conjugated IgG | Invitrogen A-21422 | RRID:AB_2535844 | 1:1000 |
| antibody | RPE65 | Novus Biologicals NB100-355 | RRID:AB_10002148 | 1:1000 |
| antibody | CRALBP | Abcam ab15051 | RRID:AB_2269474 | 1:500 |
| antibody | β-actin | Abcam ab8227 | RRID:AB_2305186 | 1:2000 |
| antibody | GFP | Invitrogen A6455 | RRID:AB_221570 | 1:5000 |
| antibody | SOX2, Tra-1–60, SSEA4, Nanog | Abcam ab109884 | 1:200 | |
| antibody | EEA1 | Fisher Scientific MA5-14794 | RRID:AB_10985824 | 1:200 |
| recombinant DNA reagent |
pEG Bacmam | other | Laboratory of Eric Gouaux | |
| recombinant DNA reagent |
pEG Bacmam-BEST1-GFP | this paper | Made from pEG Bacmam by inserting BEST1-GFP |
|
| recombinant DNA reagent |
BEST1-GFP Bacmam virus | this paper | Produced from pEG Bacmam-BEST1-GFP by published protocols (Goehring et al., 2014) |
|
| recombinant DNA reagent |
pMCSG7-10xHis-KpBestΔC11 | PMID: 25324390 | ||
| recombinant DNA reagent |
pMCSG7-10xHis-KpBestΔC11
L177T |
this paper | Made from pMCSG7- 10xHis-KpBestΔC11 by site-directed mutagenesis |
|
| recombinant DNA reagent |
pMCSG7-10xHis-KpBestΔC11
P239R |
this paper | Made from pMCSG7- 10xHis-KpBestΔC11 by site-directed mutagenesis |
|
| sequence-based reagent |
BEST1 I201T forward primer | this paper | ACCCGGGACC CTATCCTGCT |
|
| sequence-based reagent |
BEST1 I201T reverse primer | this paper | GATAGGGTCCCGGG TTCGACCTCCAAGCCACG |
|
| sequence-based reagent |
BEST1 P274R forward primer | this paper | CGCGTCTTCAC GTTCCTGCAGTT |
|
| sequence-based reagent |
BEST1 P274R reverse primer | this paper | GAACGTGAAGAC GCGCACAACGAGGT |
|
| sequence-based reagent |
KpBest L177T forward primer | this paper | ACCAGCGACA TCACTTACGGGC |
|
| sequence-based reagent |
KpBest L177T reverse primer | this paper | AGTGATGTCGCT GGTCTTGCCCGCCTCCCG |
|
| sequence-based reagent |
KpBest P239R forward primer | this paper | CGGTTTGTCTCGGTC TTTATCTCTTACACC |
|
| sequence-based reagent |
KpBest P239R reverse primer | this paper | GACCGAGAC AAACCGCGTCA TGTA GTGCAGATCGC |
|
| peptide, recombinant protein |
KpBestΔC11 L177T | this paper | Expressed from E. coli BL21 plysS, and purified by affinity and size-exclusion chromatography |
|
| peptide, recombinant protein |
KpBestΔC11 P239R | this paper | Expressed from E. coli BL21 plysS, and purified by affinity and size-exclusion chromatography |
|
| commercial assay or kit |
CytoTune-iPS 2.0 Sendai Reprogramming Kit |
Thermo Fisher Scientific A16517 | ||
| commercial assay or kit |
In-fusion Cloning Kit | Clontech 639645 | ||
| chemical compound, drug |
mTeSR-1 medium | STEMCELL Technologies 5850 | ||
| chemical compound, drug |
matrigel | CORNING 356230 | ||
| chemical compound, drug |
nicotinamide | Sigma-Aldrich N0636 | ||
| chemical compound, drug |
Activin-A | PeproTech 120–14 | ||
| software, algorithm | XDS | PMID: 20124692 | ||
| software, algorithm | Phaser | PMID: 19461840 | RRID:SCR_014219 | |
| software, algorithm | Phenix | PMID: 20124702 | RRID:SCR_014224 | |
| software, algorithm | Coot | PMID: 15572765 | RRID:SCR_014222 | |
| software, algorithm | PyMOL | http://www.pymol.org/ | RRID:SCR_000305 | |
| software, algorithm | Origin | http://www.originlab.com/index.aspx?go=PRODUCTS/Origin | RRID:SCR_014212 | |
| software, algorithm | MODELLER | PMID: 14696385 | RRID:SCR_008395 |