Table 1. In vitro BS3 X-links between subunits of E. coli RNA polymerase and 30S ribosome subunit.
Positions of X-linked residues are numbered according to full polypeptide sequences of corresponding proteins (column A) or sequences of X-linked peptides (1 being the N-terminal residue of the each peptide) (column C). Lower e-value indicates higher confidence of computational discovery of a given X-link (column B). In cases where several precursors for a given X-link were discovered (column F) the sequence of the X-linked peptide (column C), theoretical mass (column D), and experimental deviation from the theoretical mass (column E) are shown for the precursor with the lowest e-value (column B).
| X-link | Lowest e-value | Sequence of X-linked peptides and location of X-linked residues | Theoretical precursor mass (Da) | Deviation from theoretical mass (Da) | Number of precursors |
|---|---|---|---|---|---|
| RpoB(900) RS1(450) | 4.65E-010 | GETQLTPEEKLLR(10)-KGAIVTGK(1) | 2423.363414 | 0.99628 | 1 |
| RpoC(87) RS1(450) | 2.85E-007 | GVICEKCGVEVTQTK(6)-KGAIVTGK(1) | 2617.381784 | 2.99751 | 1 |
| RpoC(13) RS2(11) | 6.73E-007 | AQTKTEEFDAIK(4)-DMLKAGVHFGHQTR(4) | 3113.560294 | 3.00782 | 1 |
| RpoB(900) RS18(30) | 9.38E-005 | GETQLTPEEKLLR(10)-DIATLKNYITESGK(6) | 3202.697164 | 2.00093 | 1 |
| RpoB(900) RS21(5) | 2.75E-004 | GETQLTPEEKLLR(10)-PVIKVR(4) | 2361.363024 | −0.00541 | 2 |
| RpoC(87) RS4(185) | 1.09E-003 | GVICEKCGVEVTQTK(6)-RKPER(2) | 2529.304204 | 2.00309 | 1 |
| RpoB(265) RS19(29) | 3.12E-003 | VYVEKGRR(5)-KPLR(1) | 1655.983404 | −0.01571 | 2 |
| RpoB(476) RS1(272) | 6.47E-003 | AVKER(3)-QLGEDPWVAIAKR(12) | 2221.221774 | −0.00039 | 1 |
| RpoC(87) RS4(185) | 7.47E-003 | GVICEKCGVEVTQTK(6)-RKPER(2) | 2529.304204 | 2.00389 | 2 |
| RpoC(50) RS21(25) | 1.36E-002 | TFKPER(3)-SCEKAGVLAEVR(4) | 2232.157124 | 0.99497 | 1 |
| RpoB(265) RS19(29) | 1.50E-002 | VYVEKGRR(5)-KPLR(1) | 1655.983404 | −0.01581 | 1 |
| RpoB(909) RS21(25) | 2.25E-002 | AIFGEKASDVK(6)-SCEKAGVLAEVR(4) | 2619.357654 | 1.00524 | 1 |
| RpoC(50) RS1(434) | 8.29E-002 | TFKPER(3)-ISLGVKQLAEDPFNNWVALNK(6) | 3269.744714 | 4.00858 | 1 |
| RpoC(1192) RS16(80) | 8.79E-002 | LVITPVDGSDPYEEMIPKWR(18)-VAALIKEVNKAA(10) | 3707.984664 | 2.00023 | 1 |