Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | |||
|---|---|---|---|---|---|---|
| Recombinant DNA/adenovirus | ||||||
| FLAG-tagged SUR1 (Cricetus cricetus) in AdEasy | ham f-SUR1 | PMID: 28092267 | N/A | |||
| Kir6.2 (Rattus norvegicus) in AdEasy | rat Kir6.2 | PMID: 28092267 | N/A | |||
| tTA adenovirus | PMID: 28092267 | N/A | ||||
| FLAG-tagged SUR1 (Cricetus cricetus) in pECE | f-SUR1 | PMID: 11226335 | N/A | |||
| Kir6.2 (Rattus norvegicus) in pCDNA3 | PMID: 14707124 | N/A | ||||
| Cell lines | ||||||
| INS-1 clone 832/13 (Rattus norvegicus) | PMID: 10868964 | RRID:CVCL_7226 | ||||
| COS-M6 (Chlorocebus aethiops) | COSm6 | PMID: 11226335 | RRID:CVCL_8561 | |||
| Software/Algorithms | ||||||
| Serial EM | PMID: 16182563 | http://bio3d.colorado.edu/SerialEM | ||||
| MOTIONCOR2 | PMID: 28250466 | http://msg.ucsf.edu/em/software/motioncor2 | ||||
| CTFFIND4 | PMID: 26278980 | http://grigoriefflab.janelia.org/ctffind4 | ||||
| DoGPicker | PMID: 19374019 | https://sbgrid.org/software/titles/dogpicker | ||||
| Relion-2 | PMID: 27845625 | https://www2.mrc-lmb.cam.ac.uk/relion | ||||
| Frealign | PMID: 27572728 | http://grigoriefflab.janelia.org/frealign | ||||
| Bsoft | PMID: 11472087 | https://lsbr.niams.nih.gov/bsoft/ | ||||
| COOT | PMID: 20383002 | http://www2.mrc-lmb.cam.ac.uk/personal/pemsley/coot | ||||
| RosettaCM | PMID: 24035711 | https://www.rosettacommons.org | ||||
| UCSF Chimera | PMID: 15046863 | http://www.cgl.ucsf.edu/chimera | ||||
| Pymol | PyMOL | https://pymol.org/2 | ||||
| MolProbity | PMID: 20057044 | http://molprobity.biochem.duke.edu | ||||
| T-Coffee | PMID: 10964570 | http://www.tcoffee.org/Projects/tcoffee/ | ||||
| Chemicals/Commercial Kits/Antibodies | ||||||
| Digitonin | Calbiochem (San Diego, CA) | CAS 11024-24-1 | ||||
| ATP | Sigma-Aldrich (St. Louis, MO) | A7699 | ||||
| Glibenclamide | Sigma-Aldrich | G0639 | ||||
| QuikChange mutagenesis kit | Agilent (Santa Clara, CA) | 200515 | ||||
| FuGENE6 | Promega (Madison, WI) | E2691 | ||||
| Anti-FLAG M2 affinity gel | Sigma-Aldrich | A2220 | ||||
| FLAG peptide | Sigma-Aldrich | F3290 | ||||
| Anti-SUR1 (Oryctolagus cuniculus) | PMID: 17575084 | N/A | ||||
| Super Signal West Femto | Pierce (Rochford, IL) | PI34095 | ||||
| Other | ||||||
| R1.2/1.3 300 mesh UltrAuFoil grids | Quantifoil (Großlöbichau, Germany) | Q27507 | ||||