Table 1. Statistics of cryo-EM data collection, 3D reconstruction and model building.
| Data collection/processing | |
| Microscope | Krios |
| Voltage (kV) | 300 |
| Camera | Gatan K2 Summit |
| Camera mode | Super-resolution |
| Defocus range (µm) | −1.4 ~ −3.0 |
| Movies | 2180 |
| Frames/movie | 60 |
| Exposure time (s) | 15 |
| Dose rate (e-/pixel/s) | 8 |
| Magnified pixel size (Å) | 1.72 (Super-resolution pixel size 0.86) |
| Total Dose (e-/Å^2) | ~40 |
| Reconstruction | |
| Software | Relion and Frealign |
| Symmetry | C4 |
| Particles refined | 59,417 |
| Resolution (Relion masked) | 4.07 Å |
| Resolution (Frealign masked) | 3.63 Å |
| Model Statistics | |
| MapCC | 0.758 (masked) |
| Clash score | 9.10 |
| Molprobity score | 1.9 |
| Cβ deviations | 0 |
| Ramachandran | |
| Outliers | 0.12% |
| Allowed | 6.31% |
| Favored | 93.57% |
| RMS deviations | |
| Bond length | 0.01 |
| Bond angles | 1.11 |