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. 2017 Oct 24;6:e31804. doi: 10.7554/eLife.31804

Table 1. Primers.

See methods for usage.

ID# Name Sequence Purpose
 1 524_Bradi4g26300_4230_F CGTTCCGTGTTGATTCCGATG sopin1-1 genotyping with HgaI digestion
 2 525_Bradi4g26300_4923_R CTGGAGTAGGTGTTGGGGTTC sopin1-1 genotyping with HgaI digestion
 3 526_Cas9_8622_F TCCCAGAGAAGTACAAGGAGATCT Cas9 Genotyping
 4 527_Cas9_9159_R TTGTACACGGTGAAGTACTCGTAG Cas9 Genotyping
 5 104_BdPIN_11_QPCR_F ACAACCCTTACGCCATGAAC pin1a-1 genotyping with NcoI digestion
 6 473_PIN1a_dom1_shortR CACACGAACATGTGCAGGTC pin1a-1 genotyping with NcoI digestion
 7 541_Bradi3g59520_PIN1b_5084_F TGATGCTCTTCATGTTCGAGTACC pin1b-1 genotyping with mboI digestion
 8 542_Bradi3g59520_PIN1b_5838_R GGAGTAAACTACGTTGTGACAAGG pin1b-1 genotyping with mboI digestion
 9 019 - Ubi-1 Prom attB4 F GGGGACAACTTTGTATAGAAAAGTTGCTGCAGTGCAGCGTGACCCGG pZmUbi amplification for cloning
 10 020 - Ubi-1 Prom attB1 R GGGGACTGCTTTTTTGTACAAACTTGCTGCAGAAGTAACACCAAACA pZmUbi amplification for cloning
 11 PIN1pro-GW-F GGGGACAACTTTGTATAGAAAAGTTGTTACCCTCATCCATCATTAACTT proAtPIN1 amplification
 12 PIN1pro-GW-R GGGGACTGCTTTTTTGTACAAACTTGTCTTTTGTTCGCCGGAGAAGAGA proAtPIN1 amplification
 13 455 BdSoPIN1 cacc mRNA TCACATCTGCTGCCGCTGCC SoPIN1-Citrine coding region amplification
 14 302 - PIN_7 qPCR UTR R2 AATCCCAAAAGCCGACATTG SoPIN1-Citrine coding region amplification
 15 466 BdPIN1b cacc mRNA-2 CACCTGTACACACTGCGGCGCT PIN1b-Citrine coding region amplification
 16 308 - PIN_5 qPCR UTR R1 ACTCGCTAACCAACCCCTTAATT PIN1b-Citrine coding region amplification
 17 MVR087 - pin1-613 RP (SALK_047613) AATCATCACAGCCACTGATCC pin1-613 genotyping
 18 MVR086 - pin1-613 LP (SALK_047613) CAAAAACACCCCCAAAATTTC pin1-613 genotyping
 19 MVR036 - LBb1.3 ATTTTGCCGATTTCGGAAC pin1-613 genotyping
 20 344 - Citrine Seq R GAAGCACATCAGGCCGTAG PIN1b-Citrine and SoPIN1-Citrine genotyping
 21 524_Bradi4g26300_4230_F CGTTCCGTGTTGATTCCGATG SoPIN1-Citrine genotyping
 22 541_Bradi3g59520_PIN1b_5084_F TGATGCTCTTCATGTTCGAGTACC PIN1b-Citrine genotyping
 23 543_pin1-4_Aci_F GCTTTTGCGGCGGCTATGAGATTTGT pin1-4 genotyping with AciI digestion
 24 544_pin1-4_Aci_R GCTTCTGATTTAATTTGTGGGTTTTCA pin1-4 genotyping with AciI digestion
 25 076 - BASTA_F2 CTTCAGCAGGTGGGTGTAGAG ML1::LhG4 genotyping
 26 077 - BASTA_R2 GAGACAAGCACGGTCAACTTC ML1::LhG4 genotyping