Table 1. Primer sequences of genes selected for analysis by qRT-PCR.

Target gene	Accession number	Primer	Primer sequence (5′-3′)	Product size	Tm (°C)
NF-κB	NM_008689	Forward	GTAACAGCAGGACCCAAGGA	121bp	59
		Reverse	AGCCCCTAATACACGCCTCT
IκB	NM_010908	Forward	TGAGGACGAGGACGATAAGC	159bp	60
		Reverse	TCAGGAAGAGGTTTGGATGC
iNOS	NM_010927	Forward	GAATCTTGGAGCGAGTTGTGG	139bp	60
		Reverse	AGGAAGTAGGTGAGGGCTTGG
COX-2	NM_011198	Forward	GCCTGGTCTGATGATGTATGC	124bp	61
		Reverse	GAGTATGAGTCTGCTGGTTTGG
TNF-α	NM_013693	Forward	CACGTCGTAGCAAACCACC	88bp	59
		Reverse	TGAGATCCATGCCGTTGGC
IL-1β	NM_008361	Forward	AATGCCACCTTTTGACAGTGAT	132bp	61
		Reverse	TGCTGCGAGATTTGAAGCTG
IL-6	NM_031168	Forward	AGGATACCACTCCCAACAGACC	141bp	60
		Reverse	AAGTGCATCATCGTTGTTCATACA
IL-8	NM_011339	Forward	TTTCCACCGGCAATGAAG	115bp	59
		Reverse	TAGAGGTCTCCCGAATTGGA
IL-4	NM_021283	Forward	GTCATCCTGCTCTTCTTTCTCG	115bp	61
		Reverse	ATGGCGTCCCTTCTCCTGT
IL-10	NM_010548	Forward	GACAACATACTGCTAACCGACT	252bp	59
		Reverse	ATCACTCTTCACCTGCTCCAC
β-actin	NM_007393	Forward	GCTGTGCTATGTTGCTCTAG	117bp	59
		Reverse	CGCTCGTTGCCAATAGTG