Figure 6. Detection of mesothelin-specific circulating IgG as well as shed mesothelin protein in plasma of GBM patients.

An indirect ELISA method was used for quantifying mesothelin-specific IgG titres in plasma, while a commercially available ELISA kit was used for measuring mesothelin protein. IgG titres and mesothelin levels are expressed as ng/ml of plasma. Mann Whitney test of medians was performed to gauge statistical significance. *p < 0.05; **p< 0.001.