Table 1. N° of variations and variation rate (n° of variations/Mb) of responder and non-responder osteosarcoma patients.

Sample	N° of variations	Variations rate	Status
1	1390	118.8	Responder
5	8500	726.5	Responder
6	496	42.4	Responder
7	791	67.6	Responder
8	802	68.5	Responder
2	4211	359.9	Non-Responder
3	1495	127.8	Non-Responder
4	1095	93.6	Non-Responder

We did not find common mutated genes in all eight samples but we found three mutated genes (KIF1B, NEB, KMT2C) in 7/8 (87%) samples. Seventeen genes were mutated in 6 samples (75%, 6/8) and 57 genes showed variations in 5 samples (62.5%, 5/8). We noted a great variability of genes mutated among the samples as shown in Supplementary Table 1. Indeed, taking into consideration common mutated genes in at least 5 samples, we noted that sample n°5 showed variations in 77 genes while the sample n°6 showed variations only in 22 genes (Supplementary Table 1).

These 77 genes that were found mutated in more than half of the samples are involved in important biological processes such as transcription regulation, ion transport, DNA damage and repair, cell adhesion and angiogenesis (Table 2).