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. 2017 Aug 3;8(46):80545–80559. doi: 10.18632/oncotarget.19849

Figure 4. Macrophages isolated from GRP78 heterozygous mice have reduced cytolytic capacity.

Figure 4

(A) Protein lysates from IFNγ-treated bone marrow cells from wild-type and GRP78 heterozygous mice were analyzed for iNOS, ARG-1, and β-actin by Western blot hybridization. n = 3; *p < 0.05. (B) Protein lysates from IFNγ-treated bone marrow cells from wild-type and GRP78 heterozygous mice were analyzed for PERK, GRP78, IRE1, and β-actin by Western blot hybridization. n = 3; *p < 0.05. (C) 4T1B breast cancer cells were plated in an ACEA E-plate for 24 hours and then wild-type or GRP78 heterozygous CD11b+ cells that were pre-treated with IFNγ for 48 hours + LPS were added to the E-plate. Cell index was measured every 6 hours by electrical impedance. n = 3; *p < 0.05. (D) Signaling schematic representing how UPR targeting differentially regulates cellular bioenergetics to control macrophage polarity.