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. 2017 Aug 3;8(46):80545–80559. doi: 10.18632/oncotarget.19849

Figure 6. Dual targeting of UPR signaling in both tumor epithelial and macrophage cells affects macrophage recruitment and activity.

Figure 6

(A) IL-12p70, IL-1β, IL-6, TARC, Eotaxin, and RANTES were measured by ELISA from serum of WT and GRP78 morpholino treated mice. n = 4; *p < 0.05. (B) DMBA-induced mammary tumors from WT and GRP78 heterozygous mice (untreated or treated with tamoxifen) were stained with fluorescently labelled CD68 (green) or CD80 (red). Tumor sections were counterstained with DAPI. (C) Control, IRE1, PERK, or GRP78 transfected 4T1B breast cancer cells were plated in an ACEA E-plate and then control, IRE1, PERK, or GRP78 transfected RAW 264.7 macrophages were added to the E-plate. Each well was treated with 1 μg/mL LPS and the cell index was measured every 12 hours by electrical impedance. n = 3; *p < 0.05.