Figure 7. Resistance to CTLA-4 targeting immunotherapy modulates UPR signaling and shift macrophage polarity in human PBMC.

(A) Western blot analysis of Arg-1 and iNOS protein expression from matched human PBMC isolated from melanoma patients before Ipilimumab treatment or after progression of disease (Ipilimumab resistance). n = 5 *p = 0.02. (B) Western blot analysis of PERK, GRP78, and IRE1 protein expression from matched human PBMC isolated from melanoma patients before Ipilimumab treatment or after progression of disease (Ipilimumab resistance). n = 6; *p = 0.003 (C) Adherent PBMC from matched human PBMC isolated from melanoma patients before treatment and after ipilimumab therapy resistance were stained with CD206 M2-like macrophage marker and PERK antibodies. Cells were counterstained with DAPI.