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. 2017 Sep 19;8(46):80709–80721. doi: 10.18632/oncotarget.21080

Figure 1. EBSS induces autophagy in breast cancer cells.

Figure 1

(A and B) MCF-7 cells at 80%–90% confluence were cultured with EBSS for 0, 6, 12, and 24 h, compared with normal medium. Cells were collected for qRT-PCR to quantify the expression level of the autophagy related genes and XIAP. The error bars indicate the standard error of the mean (S.E.M) for three independent experiments (*, P < 0.05). (C) Western blot analysis of XIAP and LC3-II/I expression after MCF-7 cells treated with EBSS. (D) The gray value of P62, XIAP and LC3-II/I was calculated.