Figure 6. PP2A is required for BetA-induced apoptosis or autophagic cell death.

(A) Cells were treated by BetA for different periods of time, and then lysed for Western blotting with β-Actin serving as a loading control. Treated cells were lysed with sample buffer and subjected to immunoblot assay with an A subunit-specific anti-PP2A/A antibody. CF is referred to cleaved PP2A/A. The membrane was then stripped and reprobed with a C subunit-specific anti-PP2A/C antibody. β-Actin was used as a loading control. (B) Different cells were pre-incubated with indicated concentrations of OA for 1 h and exposed to BetA for 48 h, then lysed for detection. (C) Cells were transiently transfected with PP2A/C or Ctrl siRNA for 48 h, and then treated by BetA for Western blot analysis. (D) Cells were treated with OA or transfected with PP2A/C siRNA, and then treated with BetA for 48 h. Treated cells were collected for detection of cell viability. Graphs showing results of quantitative analyses (n=3, mean ± S.D. *,P<0.05). All data are representative of three independent experiments.