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. 2017 Sep 8;8(46):80909–80922. doi: 10.18632/oncotarget.20757

Figure 4. The WT and K472/473A mutated PFKFB3s play different roles in regulating autophagy.

Figure 4

ACHN Cells were transfected with the indicated plasmids for 36 h. (A and B) Following treatment with 6 μM rasfonin in the presence or absence of 10 μM CQ for 2 h, cell lysates were analyzed by immunoblotting with the antibodies indicated. (C) LC3 antibody was used to label the endogenous LC3, and DAPI was used to stain the nuclei. Pictures were taken by fluorescence microscopy. Numbers of the punctate LC3 in each cell were counted, and at least 30 cells were included for each group. Bar = 10 μm. (D) Suspension was collected before the treatment and lactate assay was performed. Double asterisk means p < 0.01.