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. 2017 Sep 15;8(46):80956–80970. doi: 10.18632/oncotarget.20898

Figure 6. CHERP depletion induces neuroblastoma cell apoptosis via the ER stress pathway.

Figure 6

ER stress and AKT/mTOR signaling are involved in the induction of CHERP depletion-mediated neuroblastoma cell apoptosis. (A) Western blot analysis was performed to measure DR5 protein expression. (B) DR5 mRNA levels as evaluated by qRT-PCR; the values represent the mean ± S.D. (n = 3). All data were analyzed by Student's t-test, **P< 0.001. (C) ER stress-associated proteins as analyzed by western blotting. BE(2)-C and SHEP1 cells expressing GFPsi, CHERPsi-1# or CHERPsi-2# sequences were treated with 50 μg of the ERS inhibitor GSK2606414, and the indicated proteins were detected using western blotting (D). AKT, mTOR and 4E-BP1 expression levels were also analyzed using western blotting (E and F).