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. 2017 Oct 24;7:13862. doi: 10.1038/s41598-017-14383-z

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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RNase 7 contributes to the antimicrobial activity of human stratum corneum against C. amycolatum. (a) To test the neutralizing activity of the RNase 7 antibody C. amycolatum was incubated for 3 h at 37 °C with RNase 7 (1 µg/ml; R7) together with a specific RNase 7 neutralizing antibody (R7 + R7-Ab). RNase 7 alone (R7) or together with an irrelevant antibody (R7 + irr. Ab) served as positive controls. (b) C. amycolatum was incubated for 3 h with stratum corneum extract alone (s.c.), in the presence of an irrelevant control antibody (s.c. + irr. Ab) or a specific RNase 7 antibody (s.c. + R7-Ab). After incubation serial dilutions were plated on agar-plates and the colony forming units (CFU) were determined the following day. Shown are means ± s.e.m. of five independent experiments (n.s. = not significant; **p < 0.01; ***p < 0.001, one-way analysis of variance ANOVA using Tukey’s multiple comparison test).