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. 2017 Oct 24;7:13878. doi: 10.1038/s41598-017-14118-0

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Representative composite color maps used to determine the mean blur rate (MBR) recorded by laser speckle flowgraphy (LSFG). The red color indicates a high MBR and the blue color indicates a low MBR. To measure the MBR on the optic nerve head (ONH), a circle was drawn around the ONH (A). A binary format image for segmentation between the vessel (white area) and tissue (black area) areas on the ONH (B).