Table 1.
Strains and plasmids used in this study.
| Plasmids | Description | Source |
|---|---|---|
| pTrcHis2B | trc promoter, pBR322 ori, AmpR | Invitrogen |
| pCDFDuet-1 | double T7 promoters, CDF ori, SpR | Novagen |
| pOM | Vector from Evonik (derived from pUC18), contains pGAP promoter, rrnB terminator, AmpR and ColE1 origin | [7] |
| pCA1 | pTrcHis2B carrying codon optimized R.glutinis TAL | [29] |
| pCDF-trc-RgTALsyn- Pc4CLsyn | pCDFDuet-1 carrying codon-optimized R. glutinis TAL and codon-optimized P. crispus 4CL-1 with trc promoter | [7] |
| pOM-PhCHS-MsCHI |
pOM carrying P. hybrida CHS and M. sativa CHI with a single GAP (constitutive) promoter |
[7] |
| Strains |
Description |
Source |
| P2 | E. coli K12 ΔpheA ΔtyrR lacZ::PLtetO-1-tyrAfbraroGfbrtyrR::PLtetO-1-tyrAfbraroGfbr | [24] |
| P2H | P2 hisH (L82R) (DE3) | [24] |
| PMC | P2H carrying pCDF-trc-RgTALsyn- Pc4CLsyn and pOM-PhCHS-MsCHI | This study |
| P2C | P2H carrying pCA1 and pCDFDuet-1 | This study |
| BL21 (DE3) | F−ompT hsdSB (rB–, mB–) gal dcm (DE3) | Invitrogen |
| BLNA | BL21 (DE3) carrying pCDF-trc-RgTALsyn- Pc4CLsyn and pOM-PhCHS-MsCHI | This study |
| BL21 Star (DE3) | F−ompT hsdSB (rB–, mB–) gal dcm rne131 (DE3) | Life technologies |
| StarNA | BL21 Star (DE3) carrying pCDF-RgTALsyn- Pc4CLsyn and pOM-PhCHS-MsCHI | This study |
| JM109 (DE3) | F′ traD36 proA+B+lacIqΔ(lacZ)M15/Δ(lac-proAB) glnV44 e14-gyrA96 recA1 relA1 endA1 thi hsdR17 (DE3) | NEB |
| JMNA | JM109 (DE3) carrying pCDF-trc-RgTALsyn- Pc4CLsyn and pOM-PhCHS-MsCHI | This study |
| K12 (DE3) | F- lambda- ilvG- rfb-50 rph-1 (DE3) | [7] |
| K12NA | K12 (DE3) carrying pCDF-trc-RgTALsyn- Pc4CLsyn and pOM-PhCHS-MsCHI | This study |