Table 2.

Antibodies used for immunohistochemistry

Antibody to Source		Dilution
p53	Mouse MAba (Dako, Glostrup, Denmark)	1:100
P16/INK4A	Mouse MAb (Abcam, USA)	1:500
NUPR1	Rabbit PAb (Antibodies-online Inc., Atlanta, USA)	1:500
MSH2	Mouse MAb (Merck Millipore, USA)	1:500
HSPB1/t	Rabbit PAb to hybrid Hsp25/27 (Germany)	1:500 (1:1000 for wb)
HSPB1/p	Rabbit MAb [p Ser78] (Novus Littleton, CO, USA)	1:50
Cis-DNA A	Rabbit PAb (Dr. Poirier, NIH, USA)	1:2000

^aAbbreviations used: MAb monoclonal antibody, PAb polyclonal antibody, wb Western blot, t total meaning the non-phosphorylated and phosphorylated form of the protein, p phosphorylated form of the protein using the antibody Y175, Cis-DNA A cisplatin-DNA adducts