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. 2017 Oct 24;12(10):e0186718. doi: 10.1371/journal.pone.0186718

Fig 3. miR-27a directly targets SMAD4.

Fig 3

(A) The predicted target site of has-miR-27a (middle) in the SMAD4-3’UTR region (bottom) as detected by TargetScan. A mutant SMAD4-3’UTR sequence was also shown (upper). (B) The quantification of SMAD4 protein expression in HLECs transfected with different concentrations of miR-27a inhibitor. (C) The quantification of SMAD4 protein expression in HLECs transfected with different concentrations miR-27a mimic. (D) The activity of wildtype or mutant SMAD4-3’UTR in HLECs co-transfected with control, miR-27a mimic, or miR-27a inhibitor in luciferase reporter assays. Relative luciferase activity was normalized to the scrambled oligonucleotide control. (E) The induction of the TGF-β1 pathway in HLECs transfected with control, miR-27a mimic, or miR-27a inhibitor in luciferase reporter assays. Relative luciferase activity was normalized to the scrambled oligonucleotide control. *P < 0.05, **P < 0.01.