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. 2017 Oct 24;12(10):e0186587. doi: 10.1371/journal.pone.0186587

Fig 4. Effects of T-3833261 or Halofuginone on mRNA expression of fibrosis-related genes.

Fig 4

Skin fibroblasts were pre-treated with samples (1–300 nM), followed by stimulation with TGF-β (1 ng/mL). After incubation for 24 h, α-SMA, COL1A1 (A), DDIT3, FGF2 and SMURF2 (B) mRNA levels were measured by real-time quantitative RT-PCR. Gene expression levels (normalized to GAPDH) are expressed as the fold change of vehicle-treated control. Values are mean ± SD (n = 6). #p<0.05 compared to vehicle-treated control, *p<0.05 compared to TGF-β-treated control. The experiment was repeated by using other fibroblast lots and similar results were obtained.