Table 4. Labidocera madurae (Labma) voltage-gated sodium channel transcripts/predicted proteins.
| Transcript | Deduced protein | |||||||
|---|---|---|---|---|---|---|---|---|
| Trinity ID number (Drome NaV1 hits) |
Lengthnt | Drome E-value 1 |
C. finmarchicus top hit | Labma name |
Length aa |
Type | Calfi e-value |
Flybase top hit 2 |
| TR7852|c0_g1_i1 | 7686 | 0.0 | GAXK01152315 | NaV1.1 | 1888 | F | 0.0 | para-PAL |
| TR7852|c0_g1_i2 | 7668 | 0.0 | GAXK01152315 | " | 1882 | F | 0.0 | para-PBA |
| TR7852|c0_g1_i3 | 4399 | 0.0 | GAXK01152316 | " | 1292 | N | 0.0 | para-PBA |
| TR7852|c0_g1_i4 | 2636 | 0.0 | GAXK01042242 | " | 710 | N | 0.0 | para-PBE |
| TR7852|c0_g1_i5 | 2654 | 0.0 | GAXK01042242 | " | 716 | N | 0.0 | para-PBH |
| TR7852|c0_g1_i6 | 1928 | e-168 | GAXK01042242 | " | 474 | N | 0.0 | para-PBH |
| TR7852|c0_g1_i7 | 5858 | 0.0 | GAXK01152315 | " | 1785 | N | 0.0 | para-PBA |
| TR7852|c0_g2_i1 | 6765 | 0.0 | GAXK01186590 | NaV1.2 | 2069 | F | 0.0 | para-PAL |
| TR7852|c0_g2_i2 | 1731 | e-135 | GAXK01121435 | " | 547 | I | 0.0 | para-PBE |
| TR65477|c0_g1_i1 | 3165 | 7e-89 | GAXK01056270 | NaV2 | 817 | N | 0.0 | NaCP60E-PJ |
| TR65477|c0_g1_i2 | 3220 | 7e-89 | GAXK01056270 | " | 819 | N | 0.0 | NaCP60E-PM |
| TR68660|c0_g0_i1 | 5266 | 0.0 | GAXK01056270 | " | 1755 | C | 0.0 | NaCP60E-PJ |
| TR68660|c0_g0_i2 | 5281 | 0.0 | GAXK01056270 | " | 1759 | C | 0.0 | NaCP60E-PI |
| TR25803|c0_g1_i1 | 457 | - 3 |
GAXK01114023 GAXK01037398 |
NaVX 4 | 50 | I | 4e-09 2e-08 |
para-PX |
1 Query sequence = Drosophila melanogaster canonical NaV1 sequence SwissProt P33500
2 Top BLASTp result from Flybase annotated proteins; "para" = NaV1; "NaCP60E" = NaV2
3 Original identification based on automated annotation
4 Sodium channel not fully characterized
The Drosophila melanogaster NaV1 sequence (sp|P{35500) para was used as a query in a tBLASTn probe of the Labidocara madurae 2015 transcriptome (column Drome e-value) The top hits (Trinity ID number column), with e-values < e-84, were translated into protein sequnces and reblasted using the tBLASTn tool against the Calanus finmarchicus Gulf of Maine transcriptome [23]. The top hits from that BLAST are indicated in the column "C. finmarchicus top hit," with e-values given in the column "Calfi e." These are used to identify the protein (column "Labma name") using the correspondence of comp222993 and comp299307 with NaV1.1, comp44060 and comp233807 with NaV1.2, and comp428211 with NaV2.