Fig 6. LCN parameters for WT and HDAC4/5 DKO mice.
Measurement of the number of osteocytes in a volume consisting of 100 × 100 × 10 μm3 (a), lacunar surface area (b) and lacunar volume (d) per osteocyte as well as the number of canaliculi per lacunar surface area (e) in WT and HDAC4/5 DKO mice. Data are represented as the mean ± standard deviation (error bar). A statistically significant difference was not found in the lacunar surface area and volume measurements. The lacunar surface area, lacunar volume and number of canaliculi were determined from 3D renderings generated with Imaris software where an example osteocyte in WT (c) and HDAC4/5 DKO (f) mice is shown.