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. 2017 Oct 24;12(10):e0186805. doi: 10.1371/journal.pone.0186805

Fig 2. pH dependence of V. cholerae ApbE.

Fig 2

A) Flavinylated NqrC ran through SDS-PAGE at different time points under different pH conditions. B) Kinetics of NqrC flavinilation at different pH values: pH 6.0 (Light magenta), 6.5 (magenta), 7.0 (violet), 7.5 (purple), 8.0 (dark blue), 8.25 (blue), 8.5 (cyan), 8.75 (green), pH 9.0 (yellow), 9.5 (orange) and 10.0 (red). C) Plot of the activity vs pH. Data were fitted using Eq 1. D) Effects of 0.5 mM and 1mM FAD on the reaction rate obtained at pH 6, 7, and 9 (n = 3). E) Effects of pH on ApbE stability. ApbE samples were preincubated for 10 and 30 min at pH 5.5, 6.0 and 9.0. The samples were diluted (1:20) in pH 9.0 buffer (Tris 100 mM) and the flavin transfer activity was measured in the same buffer (n = 3).