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. 2017 Oct 24;12(10):e0186891. doi: 10.1371/journal.pone.0186891

Fig 1. Plasmid maps of pCNA, pSNK, and pBDC.

Fig 1

The helper plasmids pCNA and pSNK, which could be eliminated by incubation at 42°C, were obtained upon insertion of I-CreI and I-SceI, respectively, into pKOBEG. The bla cassette was obtained from pET3b (Novagen), and the kan cassette was obtained from pET28a (Novagen). The p15A ori and cat cassettes of the landing pad plasmid pBDC were obtained from pACYCDuet-1 (commercial plasmid purchased from EMD Biosciences). IC and IS represent the I-CreI endonuclease recognition site and I-SceI endonuclease recognition site, respectively.