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. 2017 Oct 1;36(5):199–207. doi: 10.1089/mab.2017.0044

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Copyright 2017, Mary Ann Liebert, Inc.

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FIG. 5. — Quantitation of picrosirius red-stained collagen fibrils present in the ROIs corresponding to the fibrotic lesions of the lungs from BL-treated mice. (A, B) Representative images of a readily visible FL (delineated) observed in normal light (A) and polarized light (B). (C) High-magnification view of collagen fibrils seen in the corresponding region (asterisk) in (B). (D) Graphic representation of the area occupied by collagen fibrils present in the lesions of the hIgG-treated mice, AFA-treated mice, and nontreated mice (Ctrl). (E) A graphic illustration of the relationship between the size of lesions and the density of collagen fibrils. Asterisks indicate statistically significant differences between analyzed groups: **p < 0.01, ****p < 0.0001. Scale bars = 50 μm. BL, bleomycin; FL, fibrotic lesion; ROI, region of interest.