Table 1. Primer sequences for specific genes.
| Gene | Primer sequence |
|---|---|
| ANP | F: 5'-ACCTGCTAGACCACCTGGAG-3' |
| R: 5'-CCTTGGCTGTTATCTTCGGTACCGG-3' | |
| BNP | F: 5'-GAGGTCACTCCTATCCTCTGG-3' |
| R: 5'-GCCATTTCCTCCGACTTTTCTC-3' | |
| β-MHC | F:5'-CCGAGTCCCAGGTCAACAA-3' |
| R: 5'-CTTCACGGGCACCCTTGGA-3' | |
| MCIP1.4 | F: 5'-TCCAGCTTGGGCTTGACTGAG-3' |
| R: 5'-ACTGGAAGGTGGTGTCCTTGT-3' | |
| Collagen1α | F: 5'-AGGCTTCAGTGGTTTGGATG-3' |
| R: 5'-CACCAACAGCACCATCGTTA-3' | |
| CollagenIII | F: 5'-CCCAACCCAGAGATCCCATT-3' |
| R: 5'-GAAGCACAGGAGCAGGTGTAGA-3' | |
| Fibronecin | F: 5'-CCGGTGGCTGTCAGTCAGA-3' |
| R: 5'-CCGTTCCCACTGCTGATTTATC-3' | |
| MMP-2 | F: 5'-TTTGCTCGGGCCTTAAAAGTAT-3' |
| R: 5'-CCATCAAACGGGTATCCATCTC-3' | |
| GAPDH | F: 5'-ACTCCACTCACGGCAAATTC-3' |
| R: 5'-TCTCCATGGTGGTGAAGACA-3' |
ANP, atrial natriuretic peptide; BNP, brain natriuretic peptide; β-MHC, β-myosin heavy chain; MCIP1.4, modulatory calcineurin-interacting protein1.4;MMP-2, matrix metalloproteinase-2; GAPDH, glyceraldehyde-3-phosphate dehydrogenase.