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. 2017 Sep 12;292(42):17178–17189. doi: 10.1074/jbc.M117.796045

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© 2017 by The American Society for Biochemistry and Molecular Biology, Inc.

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Figure 2. — NUSAP1 interacts with the RanBP2–RanGAP1–UBC9 complex in a cell cycle-dependent manner. A, Venn diagram showing overlap of IP-MS/MS experiment results. B, TSC for each of the RanBP2–RanGAP1–UBC9 complex members determined by mass spectrometry. C, endogenous NUSAP1 IPs were performed in four different nocodazole-arrested cells and analyzed for RanBP2. D, endogenous RanBP2 IP performed in nocodazole-arrested 293T cells. E, size exclusion chromatography was performed on extracts from nocodazole-arrested 293T cells. Extracts were analyzed on a Superose 6 column. Previously tested size markers migrated in the indicated fractions. F, endogenous NUSAP1 IPs were performed using each of the gel filtration fractions from E. *, indicates SUMOylated RanGAP1.