Figure 3.

NUSAP1 depletion does not affect mitotic localization of the RanBP2–RanGAP1–UBC9 complex. A, endogenous RanBP2 and RanGAP1 localization in both interphase and metaphase HeLa cells. B, endogenous RanBP2 localization in either control or NUSAP1-depleted HeLa cells. C, endogenous RanGAP1 localization in either control or NUSAP1-depleted HeLa cells. D, chromatin fractionation in U2OS cells. Cells were transfected with either control or NUSAP1 targeting siRNA and split for overnight treatment with either DMSO or nocodazole. (WCE = whole cell lysate; S = soluble (cytoplasmic); I = insoluble (nuclear/chromatin). All scale bars indicate 10 μm.)