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. 2017 Sep 7;292(42):17190–17202. doi: 10.1074/jbc.M117.799650

Figure 6.

Figure 6.

A novel EVL isoform lacking the zDABM sequence is unable to interact with the AR domain of zDHHC17. A, domain architecture of EVL isoforms 1 and 5 (WH1, WASP-homology 1; EVH2, Ena/VASP-homology 2). Isoform 5 is missing a 21-amino acid region (shown in red) containing the zDABM sequence (amino acid sequence of this stretch is shown with the critical for ARzD17-binding positions indicated in red). B, EGFP-tagged EVL-1 and EVL-5 human proteins were expressed in HEK293T cells, and their ability to interact with the AR domain of zDHHC17 was assessed by pull downs of corresponding lysates by ARzD17-His (51–304 amino acids of human zDHHC17). 7.5% of total inputs and 40% of total bound fractions were run on 12% gels, and following transfer, blots were stained by Ponceau S solution and probed with a GFP antibody.