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. 2017 Sep 7;292(42):17250–17257. doi: 10.1074/jbc.M117.813899

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© 2017 by The American Society for Biochemistry and Molecular Biology, Inc.

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Figure 5. — Regulation of TRAF6 polyubiquitination by OPTN. A, HEK293T cells were transfected with FLAG-tagged TRAF6 and HA-tagged ubiquitin with or without Myc-tagged OPTN. After 36 h of transfection, immunoprecipitation (IP) was performed using anti-FLAG antibody. Then Western blotting (WB) was performed using anti-FLAG, anti-HA, or anti-Myc antibody. Data are representative of three independent experiments with similar results. B, relative polyubiquitination level of TRAF6 in OPTN-transfected HEK293T cells. Data are the mean ± SD of three independent experiments and are presented relative to the vector-transfected control cells, set as 1. *, p < 0.05.