Table 1.

Electrostatic and hydrogen bond distances in the PhyAsrC252S·Ins(1,3,4,5)P₄, PhyAsrC252S·Ins(1,4,5)P₃, and PhyAmmC250S/C548S·Ins(1,3,4,5)P₄ structures are highly similar

Contact distances (<3.4 Å) between PhyA and the ligand phosphoryl (Phos) and hydroxyl (-OH) groups are shown. Bolded distances are main-chain interactions.

Residue	Site	PhyAsrC252S				Residue	Site	PhyAmmC250S/C548S
		Ins(1,3,4,5)P4		Ins(1,4,5)P3				Ins(1,3,4,5)P4
		Phos/-OH	Distance (Å)	Phos/-OH	Distance (Å)			Phos/-OH	Distance (Å)
Arg-57	Pa′	P3	2.80/3.17			Arg-351	Pa′	P3	3.16/2.85/2.64
Asp-153	Pa′					Asp-449	Pa′	P3	3.24
Lys-189	Pa′	P3	2.60			Lys-485	Pa′	P3	2.73
	Pc	P4	2.96	P4	2.33		Pc	P4	3.07/3.03
Asp-223	Pa′	P3	3.16/2.81			Asp-519	Pa′	P3	2.67
His-224	Pa	P3	2.95			His-520	Pa	P3	3.02
		P4	3.4
		P5	2.51
Ser-252	Ps	P1	2.51	P1	2.27	Ser-548	Ps	P1	2.67
Glu-253	Ps	P1	3.06	P1	3.00	Gln-549	Ps	P1	3.22
Ala-254	Ps	P1	3.17	P1	3.24	Ala-550	Ps
Gly-255	Ps	P1	2.91	P1	2.79	Gly-551	Ps	P1	3.15
Val-256	Ps	P1	2.69	P1	2.92	Ala-552	Ps	P1	2.90
Glu-257	Pa	O6	3.16	O6	3.08	Gly-553	Pa	O6	3.34
								P1	3.31
Arg-258	Ps	P1	2.92/3.00/2.99	P1	2.72/2.90/2.90	Arg-554	Ps	P1	2.73/3.11/2.76
Lys-305	Pc	P4	2.52/2.51	P4	2.41	Lys-600	Pc	P4	2.90/3.10/3.15
	Pb	P5	2.82	P5	3.02		Pb	P5	2.95/2.99
Tyr-309	Pb	P5	2.82/2.47/3.18	P5	2.68/3.13	Tyr-604	Pb	P5	2.31/3.20/3.38