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. 2017 Aug 31;292(42):17337–17350. doi: 10.1074/jbc.M117.805036

Figure 2.

Figure 2.

The Arg-GlcNAcylated host protein repertoire during EPEC infection is predominantly restricted to FADD. A, time course of EPEC E2348/69 infection over 12 h shows the appearance of a single dominant band consistent with the size of FADD (∼25 kDa). B, extracted ion chromatograms showing the presence of modified FADD peptides at 3 h postinfection with wild-type EPEC E2348/69 that are absent in the Arg-GlcNAc enrichment of EPEC E2348/69 ΔPP4/IE6 (pNleB1DXD)-infected cells 3 h postinfection. EThcD fragmentation confirms that the site of modification in FADD corresponds to Arg117. HexNAc, N-acetylhexosamine.