Table 1.

Purification of human cis-PT from Expi 293 cells

Mean ± S.D. values of three replicates are shown for specific activity.

Step	Total protein	Total activity	Specific activity	Yield	Purification fold
	mg	μmol	μmol/h/mg protein	%
Crude extract	240.24	6.32	0.023 (±0.003)	100
Membrane pellet	140.7	2.20	0.016 (±0.002)	34.8	0.7
SUP	71.45	2.14	0.030 (±0.001)	33.9	1.3
S-Tactin	0.06	1.45	24.13 (±4.91)	22.9	1049
Nickel column	0.024	0.98	40.97 (±9.39)	15.5	1781