Table 2.

Kinetic parameters of human cis-PTs

Mean ± S.D. values of three replicates are shown.

	Km		kcat
	IPP	FPP
	μm	μm	1/s
hcis-PT, WT	11.1 (±1.3)	0.68 (±0.14)	0.58 (±0.02)
hcis-PT, hCIT K42E	9.4 (±2.1)	2.59 (±0.43)	0.12 (±0.03)
hcis-PT, NgBR H100A	14.9 (±2.0)	0.89 (±0.32)	0.16 (±0.01)
hcis-PT, NgBR R290H	24.8 (±4.4)	1.64 (±0.32)	0.12 (±0.01)
hcis-PT, NgBR G292A	57.7 (±7.4)	0.19 (±0.06)	0.05 (±0.002)