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. 2017 Aug 29;292(42):17407–17417. doi: 10.1074/jbc.M117.804377

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Figure 5. — LmeA is a cell-envelope protein. A, immunoprecipitation (IP) of LmeA-HA with (+) or without (−) a mild detergent. Western blotting using anti-HA antibody showed that anti-HA immunoprecipitation of LmeA-HA was possible only when Triton X-100 was present during the incubation. Two separate strains expressing either LmeA-HA (29.5 kDa) or a cytosolic marker, GlnA1-HA (54.6 kDa), were used. B, sucrose gradient sedimentation of LmeA-HA expressed from the native promoter. Expected molecular masses were as follows: anti-HA, LmeA-HA (29.5 kDa); anti-MptC, cell-envelope marker (48.2 kDa); anti-PimB′, intracellular membrane domain (IMD) marker (41.4 kDa); and anti-Mpa, cytosolic marker (68.0 kDa).