Figure 3.
Localization of MS-identified in situ phosphorylation sites on the mouse ventricular NaV1.5 α subunit protein. Among the 19 phosphorylation sites identified, 10 (in blue) had already been identified in our previous MS analyses and 9 (in red) are novel. Four and two phosphorylation site locations are possible at amino acids 36–42 and 524–525, respectively. The three newly identified C-terminal phosphoserines at positions 1888, 1937, and 1938 (pSer-1888, pSer-1937, and pSer-1938) are in close proximity to the binding sites for iFGF and calmodulin (IQ-motif).