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. 2017 Sep 7;292(42):17431–17448. doi: 10.1074/jbc.M117.787788

Figure 6.

Figure 6.

Phosphorylation at serines 1933 and 1984 disrupts the FGF13-dependent decrease in INaL. TTX-sensitive late Na+ current (INaL) recordings were obtained 48 h after transfection of HEK293 cells in three different data sets. The first data set (A and B) was obtained from cells expressing NaV1.5-WT (black), NaV1.5-WT + FGF13 (green), and NaV1.5-S1933E/1984E + FGF13 (NaV1.5-EE + FGF13, red); the second data set (C and D) from cells expressing NaV1.5-WT (black), NaV1.5-WT + FGF13 (green), and NaV1.5-S1933A/S1984A + FGF13 (NaV1.5-AA + FGF13, blue); and the third data set (E) from cells expressing NaV1.5-WT (black), NaV1.5-S1933E/S1984E (NaV1.5-EE, purple), and NaV1.5-S1933A/S1984A (NaV1.5-AA, pink). A and C, representative TTX-sensitive INaL recordings evoked by prolonged depolarizations (350 ms at −20 mV) from a holding potential of −120 mV. The scale bars indicate a current amplitude of 10 pA, which corresponds to a current density of 0.8 pA/pF and time (50 ms). B, D, and E, distributions and mean ± S.E. TTX-sensitive late Na+ current (INaL) densities. *, p < 0.05; ***, p < 0.001 versus NaV1.5-WT; #, p < 0.05 versus NaV1.5-WT + FGF13; ns, non-significant; Kruskal-Wallis one-way ANOVA followed by the Dunn's post hoc test. Detailed densities and statistics are provided in Table 3.