Table 1.
Phosphorylation sites, phosphopeptides, and site-discriminating ions identified in immunoprecipitated NaV1.5 α subunits from adult WT and/or CaMKIIδc-Tg mouse ventricles using MS
The site-discriminating ions observed in the high-resolution (LTQ-Orbitrap Elite or TripleTOF 5600 Plus) or low-resolution (LTQ-Orbitrap XL) MS/MS spectra of each annotated NaV1.5 phosphopeptide support the assignment of the indicated phosphorylation site(s). The charge state as well as the percentage of maximum intensity of each observed unphosphorylated and phosphorylated site-discriminating (b and y) ion are reported in parentheses; the minus symbol indicates that the charge state could not be determined.
