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. 2017 Sep 12;292(42):17461–17472. doi: 10.1074/jbc.M117.810960

Figure 2.

Figure 2.

Plk1 detection in the tissues of double transgenic mice (CMV-Cre/Plk1-KI). A, relative Plk1 mRNA levels in the tissues of CMV-Cre/Plk1-KI animals were compared with the levels in Plk1-KI but without Cre expression (Control, Ctrl) animals (mean ± S.D., n = 3 for each tissue). B, total cellular protein was prepared from the indicated tissues of adult CMV-Cre/Plk1-KI or control mice and subjected to IB. C, animals were sacrificed at the age of 1 month, followed by anti-Plk1 IHC staining of indicated tissues. Sections representing the organs of control (Plk1-KI only) and CMV-Cre/Plk1-KI mice. Bar, 200 μm. D, top panel, whole cell extracts from MEFs isolated from CMV-Cre/Plk1-KI and control animals were subjected to IB. Bottom panel, PCR of mouse genomic DNA (tail clips) are presented. E, relative Plk1 mRNA levels in the tissues of Myod-Cre/Plk1-KI animals were compared with the levels in control animals (mean ± S.D., n = 3 for each tissue). F, total cellular protein was prepared from the indicated tissues of Myod-Cre/Plk1-KI and control mice. **, p < 0.05; ***, p < 0.01.