Figure 4.

TonEBP regulates TNF-α-dependent expression of select genes linked to matrix catabolism. A, qRT-PCR confirmation of TonEBP knockdown. TonEBP and TauT mRNA levels were unaffected by TNF-α treatment in ShControl-transduced cells but decreased in cells transduced with ShTonEBP. B, TonEBP regulated transcript levels of matrix catabolic enzymes ADAMTS4, ADAMTS5, and MMP3 under TNF-α treatment. C, TNF-α treatment significantly decreases transcript levels of ACAN and COL2A1 in ShControl-transduced cells. Levels were unaffected by knockdown of TonEBP. Quantitative measurements represent mean ± S.E. of ≥3 biological replicates. *, p ≤ 0.05; **, p ≤ 0.01, ****, p ≤ 0.0001. ns, not statistically significant.